Sentence examples for a step of template from inspiring English sources

In brief, total RNA was reverse transcribed to first stand cDNA coupled with (dC) tailing and CDS III primers (both containing SfiI digestion site sequences) following a step of template switching and extension by reverse transcription.

Alternately, colony PCR can be performed by adding a DNA polymerase with a PCR master mix to a lysed cell sample from a single recombinant colony, omitting the step of template purification.

The input is the same as before: a content sequence and a set of templates; At each step, every template is tested against the current sequence of content, in order to evaluate how much content can be consumed by this template; The template that uses the most content with the least amount of error is selected and the sequence of content is reduced.

By adding a PNKP-triggered 5′-phosphroylation step of the template DNA, the above sensing strategy could be easily extended to the design of PNKP sensor.

Our method is a step toward the template-free discovery of the shapes, abundance and spatial distributions of previously unknown macromolecular complexes in whole cell tomograms.

The final step of the template fabrication is the electric passivation of the pore walls by an 8-nm-thick layer of Al2O3 deposited by atomic layer deposition (ALD) to avoid unfavorable current flow through the pore walls during galvanic deposition.

The thermal cycle conditions were: denaturation at 95°C for 10 min, followed by 35 three-step cycles of template denaturation at 95°C with a 2 s hold, primer annealing at 60 to 65°C for 15 s, and extension at 72°C for 60 s/1000 bp.

The thermal conditions for qPCR were as follows: denaturation at 95°C for 10 min, followed by 35 three-step cycles of template denaturation at 95°C with a 2 s hold, primer annealing at 60°C for 10 s, and extension at 72°C for 20 s.

The following standard thermal profile was used: denaturation at 95°C for 10 min, followed by 35 three-step cycles of template denaturation at 95°C with a 5 s hold, primer annealing at 58-64°C 58-64°Cs and extension at 72°C for 10 s.

The thermal cycle conditions were: denaturation at 95°C for 10 min, followed by 35 three-step cycles of template denaturation at 95°C with a 2 s hold, primer annealing at 60 65°C for 15 s and extension at 72°C for 25 s.

However, such methods still have the drawback that they are either restriction enzyme-dependent, Phi29 DNA polymerase-dependent, or require a series of template DNA purification steps [ 2, 6, 17].