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The NMR models of these lariat RNAs provide a starting model from which several structural features can be characterized.
We built a starting model from F-actin with a talin dimer attached in arbitrary orientation close to the centre of the image.
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Upon the basis of a starting model, derived from the PaDDAH crystal structure from which the l-citrulline has been removed, the unbiased MD simulation of DM-PaDDAH provides a visual representation of the nanosecond time scale dynamics of the protein.
The best-fitting model of the source parameters (M o, f c, t g * ) was obtained by iteration from a starting model using the simplex algorithm (Nelder and Mead 1965; Caceci and Cacheris 1984, Numerical Recipes 1998, Chap. 10.4).
Initial electron density maps were calculated by molecular replacement using Phaser McCoyy et al., 2007) from a starting model of Taq Δ1.1σA-holoenzyme determined at 2.8 Å-resolution (unpublished).
Structure solution and refinement were performed from a starting model based on ZIF-7-I in P1 symmetry using rigid bodies, combined with energy minimization, to arrive at the final P1̅ structure.
To accommodate a second producer, the starting model from subsection "Model geometry and properties" is extended along the direction of maximum compressive stress σ 1 with an i-p separation of 2000 m while keeping the thermal and hydraulic properties the same.
This procedure, e2refine-evenodd, randomizes the phases of the starting model from a user-defined cut-off resolution and then refines two half-data sets against this model.
Overview of the estimation approach: 1. Estimate a starting ρ from the length model by maximum likelihood.
The first month of data available on friendships is used as a starting point for the model; data from September is used for model initialization.
However, the creation of such a model starting from sequential code is a time-consuming and error-prone task.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com