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That is a standard reaction of knowledgeable scientists.
Alternatively, the GSH could be reacting directly with the IFNQ as this is a standard reaction of quinones and reduced glutathione [ 33].
Finally, to assess the inhibition of SAMHD1, each compound was added to a standard reaction of GTP-activated SAMHD1 at a concentration equimolar to the allosteric activator (200 μM), with 1 mM TTP as the substrate (Fig. 4D).
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Reducing sugars are tested by Benedicts and Fehling's reagents which involve the reduction of Cu2+ to Cu+ which has a standard reaction potential of +0.16 V [54].
Generally, a standard reaction consists of methanol in a 6 1 molar ratio with oil input, 1 100 molar ratio of NaOH to oil input, and a 1 1 volume ratio of organic solvent to methanol [12].
1 Inhibition assays were performed using a standard reaction mixture of 50 mM potassium phosphate buffer (pH 7.5), 12 mM MgCl2, 50 μM DTP, 5 10 times the Km value of the nonvaried substrate (AcCoA or KIV), and varied concentrations of leucine (0 120 μM).
The coupled enzyme assay of C-654 was conducted in a standard reaction mixture consisting of C-654 (0.03 mg), PEP (0.7 mM), PK (2 U), probe (2 μL), and enzyme mix (2 μL) in a total volume of 100 μL.
Only one of the interviewees (an organizer) was explicitly aware of an evaluation that directly influenced future conference development and none of the stakeholders had ever reported participating in any type of assessment beyond a standard reaction evaluation at the end of the conference.
A standard reaction sample was composed of a mixture containing enzyme and 0.5% (w/v) β-glucan incubated in 10 mM NaPO4 buffer, pH 7.0.
A standard reaction mixture contained 100 ng of sample DNA, one set of primers and the SYBR Green I PCR master mix (Applied Biosystems) at a total volume of 25 μl.
Assessment criteria have been provided by the study of the kinetics of a standard reaction-dissociation of carbon dioxide.
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