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Each crude extract and corresponding set of nine fractions (10 samples total per strain) were concentrated to dryness, weighed, brought to a standard concentration of 25 mg/ml in DMSO, and dispensed (10 µl) into 96-well microtiter plates.
All extracts were concentrated under vacuum until dryness and re-dissolved to a standard concentration of 5 mg ml-1 in MeOH.
A standard concentration gradient which simulates plant living cells was produced by an electrode with a certain tip diameter, filled with a solution containing a known K+ concentration in 100 mmol/L.
A standard concentration of 5 µg/ml SOR (internal standard) was added to the solutions.
The technique was based on the inhibitory activity of discs (Oxoid, UK) containing a standard concentration of cefazolin.
Twenty millilitres of the metal ion solution with a standard concentration of 60 ppm from Pb NO3 2 was transferred to a beaker containing the weighed nanocomposite.
Twenty millilitres of the metal ion solution with a standard concentration of 60 ppm from Pb NO3 2 solution was transferred to each beaker containing the weighed nanocomposite.
AIG was supplied by the CDC (lot number 10602912, Cangene Corporation, Winnipeg, Canada) at a standard concentration of 2.18 mg/mL in 50-mL vials.
The fungus was grown on Potato dextrose agar slants and selected based on its virulence against tomato insect pest complex through laboratory bioassay studies with a standard concentration of 1 × 108 spore's ml−1.
The supernatant was removed and the bacterial pellet was re-suspended in a saline solution (0.85% NaCl) and then diluted to a standard concentration equal to an optical density (OD) of 1.0 at 545 nm.
A standard concentration curve for each cDNA was constructed from serial dilutions of linearized plasmid DNA.
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