Sentence examples for a standard buffer from inspiring English sources

The vitrification medium comprised a standard buffer for fish spermatozoa (Cortland®medium) + 10% DMSO + 2% BSA + 0.13 M sucrose + SP at concentrations of 30% (group 1), 40% (group 2) or 50% (group 3).

The vitrification medium consisted of a standard buffer for fish spermatozoa (Cortland medium) + 10% DMSO + 2% BSA + 0.13-M sucrose + SP at concentrations of 30% (G30), 40% (G40), or 50% (G50).

Since [H3O+] must also be known, the commonest procedure is to measure [A]/[B] in a solution made by adding a small quantity of A or B to a standard buffer solution.

For the Glu treatments, Fura-2 loaded HEK293 cells were incubated in a standard buffer (pH 7.4) containing 130 mM NaCl, 3 mM KCl, 0.6 mM MgCl2, 10 mM glucose, 10 mM HEPES and 1 mM Ca2+ for 30 min.

This sort of database can only be reliably used if the quality of the datasets is assured by eliminating chemical shift data that have not been generated in a standard buffer and at a constant temperature.

Experiments were conducted when the cultures reached 100% confluence, after trypsinization and resuspension of the cells in a standard buffer (pH = 7.4) containing (in mmol/L): NaCl, 137; Hepes, 5; glucose, 22; taurine, 20; creatine, 5; KCl, 5.4; MgCl2, 1; sodium pyruvate, 5 and CaCl2, 1 [14] [14].

The protocol for composing this buffer is sufficiently complex that "homemade" buffers (i.e., buffers constructed using the prescribed seawater buffer recipe) should "...be calibrated against a primary standard buffer whenever practical".

A standard bicarbonate buffer was used as dialysate buffer.

Both the peptide and the mimetic had been dissolved in standard buffer A containing 1 m m MgCl2 and 1 m m MnCl2.

Figure 2 figure supplement 4 shows raw decay time data from DLS for FG N and FSFG-K constructs in standard buffer A, 8 mg/ml.

After establishing a baseline with the standard buffer increasing concentrations of mucin were run over the chip surface at a flow rate of 10 μL/min for 10 min each followed by a 15 μL/min 10 min rinsing step.