The phrase "a stack of reading" is correct and usable in written English.
It can be used to refer to a collection of reading materials, such as books, articles, or papers that one needs to go through.
Example: "I have a stack of reading to get through before the end of the week for my literature class."
Alternatives: "a pile of reading" or "a heap of reading".
If a sequencing or mapping error was the cause of variation, two or more variants were expected at a nucleotide position in a stack of reads, and the frequency of one of the variants was expected to be minor.
If heterozygosity was the cause of variation, two variants were expected at a nucleotide position in a stack of reads, and the expected frequency of each variant was 0.5.
FVF represents the frequency of minor read variants in a stack of reads, which follows a binomial distribution and is expected to be 0.5 in a random mating population.
Hence, observing two different nucleotides at a nucleotide position in a stack of mapped reads should be used as evidence for an SNP.
Tessa Hadley's previous novel, The London Train, featured a scene in which the male protagonist considers the stack of reading material on his wife's side of the bed: "Novels which seemed to him pretty interchangeable – what people called 'women's fiction'".
Typically, the stack of reading material on her nightstand would include a mix of manuscripts from work, novels from her many book clubs and plenty of nonfiction, most recently books on American constitutional law.
Now, members of the new Southern Tier network have added to the governor's stack of reading.
For reasons to tedious to get into, the book wound up in a stack of backlogged reading, and I'd only got round to reading it after the interview in the current Quarterly Conversation.
A special case was when FVF > 0 but the stack of reads varied for two nucleotides, both differing from the reference sequence.
Instead, he must face a stack of assignments, and read and grade them on his own time.
