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Following mounting, the perfused vessels were allowed to equilibrate and attain a stable tone.
Once a stable tone was established, a dose response curve was constructed by administration of bolus doses of tryptamine, tyramine or β-PEA (0.1 1000 nmoles/100 µL) via an injection port.
After a stable tone had been reached (∼20 min), cumulative concentration response curves were established in separate rings with GTN (0.1 nM–300 μM) or DEA/NO (1 nM–10 μM).
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Although standard fingering generates a brighter and more stable tone than an overblown sound, alternative fingerings are frequently used to minimize mistakes in note transition or to add a special color to the tone, especially in contemporary repertoire [3].
But I don't think he ever found a stable visual tone for the Rings trilogy: Frodo didn't need his magic sword to tell when Orcs were close by – Jackson's off-kilter camera told him everything he needed to know.
It's best to play a fifth above the tonic as the fifth is always the second most stable tone in any key.[8].
The vessel segments were once again allowed to equilibrate (i.e. attain stable tone) before any further experiments were conducted.
After development of stable tone, intravascular flow was re-established from almost static conditions (∼30 µl/min) to physiologically relevant levels (600 µl/min) by increasing the pressure gradient between inflow and outflow capillaries to 20 mmHg.
Germanic and English-speaking countries tend to use a moveable do, such that do refers to the tonic (i.e., the most stable tone) of any scale (e.g., C in the key of C, C-sharp in the key of C-sharp, and so on).
Additional analyses show that these postures reach their achievement point at a stable temporal distance from boundary tone onsets regardless of stress position.
After the stabilization of resting tone, a solution of either phenylephrine (Sigma-Aldrich, St . Louis MO, USA) or KCl prepared in distilled water was added to the bathing buffer to induce a marked raise in vascular tone followed by a stable vasoconstriction (tonic contraction).
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