I then paired females socially with a non-desired mate and once they had formed a stable pair bond, I observed the inclination of these females to engage in extra-pair copulations with various males.
The most stable pair of internal controls was a combinations of two genes, U6 and U91 (stability value = 0.036; Table 5).
The results of geNorm and Normfinder are shown in Figure 3. geNorm, which treats all data as a homogenous group, identified PDA1 and IPP1 as the most stable pair of genes and ACT1 as the 3rd best reference gene candidate.
Atthis threshold, the semi-classical system undergoes a supercritical pitchforkbifurcation where the stable origin goes unstable, and the stable pair of fixedpoints emerges from the origin and grows in separation with increasing parametricpumping (the checkered red region in the upper half-plane in Figure 3).
In a previous study, miR-191 and miR-25 were identified as the most stable pair of normalisers across 13 distinct human tissue types including 5 pairs of colon tumour and adjacent normal tissues.
The spatial discretization is performed with P2−P1 finite elements but any other stable pair of approximations can be used.
Both programs selected miR-16 and miR-345 as the most stable pair of reference genes.
SCS = Standard Curve Slope The most stable pair of reference gene primers were selected using the normalization strategy proposed by [ 33].
GeNorm identified GapDH and ACTB to be the most stable pair of reference genes (M = 0.127, V = 0.048) (Table 3).
Using geNorm, we defined PTB1 and GAPDH as the most stable pair of reference genes for RT-qPCR.
To test the robustness of these two genes across different treatments, we compared the normalization factors calculated based on RPL19 and RPL29 (NFRPL19/RPL29) to that of the most stable pair of reference genes (NFtop2) in each treatment subgroup.
