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These cells were engineered so as to support a stable expression of human cyclin T1 and were further transduced with HIV-1 gag and pol genes.
Although E6 gene is transcribed through bicistronic E6/E7 mRNA, studies show that transfection with monocistronic E6 into C33A cells could also result in a stable expression of unspliced E6 mRNA (del Moral-Hernandez et al., 2010).
(4) The donor cells for SCNT are selected by G418, enabling to integration of the four genes into the genome, resulting in a stable expression of transgenes throughout the whole lifetime of transgenic pigs.
When cultured in Defined medium, HDS had a stable expression of DDR2 over time.
We established a stable expression of CXCL16 in SL4, a metastatic mouse CRC cell line, and confirmed its expression.
Our transcriptome data also indicated a stable expression of EEF1A among all samples (data not shown), as expected for an internal control housekeeping gene.
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While this approach leads to a stable expression pattern, the generation of transgenic mice is expensive and time consuming.
It was therefore necessary to establish a stable expression system for production of recombinant B3 and its variants in Chinese hamster ovary (CHO) cells.
Importantly, in particular for clinical applications of genome-engineered cell products, it will also be of importance to remove those cells that, due to random vector integration, display an unwanted stable expression of the auxiliary gene.
These data suggest that a vector-based stable expression of shRNA molecules induces a weaker interferon system response with respect to the transfection of synthetic siRNAs.
Second, we describe a method for stable expression of genes of interest in trichomes.
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