Sentence examples for a stabilization solution from inspiring English sources

The crystals were stabilized and cryoprotected by being transferred first to a stabilization solution containing 100 mM sodium cacodylate buffer (pH 6.5), 20% (w/v) PEG350 MME, and 100 mM CaCl2.

After the reaction at room temperature for 5 min, the brainstems were soaked in a stabilization solution (ELISA POD substrate TMB kit HYPER) at 4°C for 20 min. Immediately after the stabilization, the pathways of HRP-filled retinal fibers running on the surface of the brainstems were photographed using a stereomicroscope (MZ16F; Leica Microsystems).

A stabilization solution (200 mg of sodium ascorbate and 30 mg of DTT in 1 ml of water) was then added and samples were stored at − 20 °C until analysis.

Crystals were transferred to a stabilization solution of 4.8% polyethylene glycol 4000 in the same buffer for 1 hour, then transiently (<30 sec) placed in a cryoprotectant of 30% polyethylene glycol 400 and 6% polyethylene glycol 4000 in 50 mM potassium MES (pH 7.0) and flash-frozen in liquid N2 for data collection.

At the end of the experiment, the leading shoot from three plantlets of each of the three lines tested were sampled and pooled in Kennedy Space Center Fixation Tubes KFTT) containing a RNA stabilization solution.

Fragments of liver tissue (5 7 mm) were immediately frozen with a RNA stabilization solution (RNAlater® solution, Life Technologies, Grand Island, NY, USA) in liquid nitrogen and stored at − 80 °C until RNA extraction.

To minimize the potential impact of RNA degradation on microarray data, resected tissue should be sectioned and either flash frozen or immersed in a tissue stabilization solution such as RNALater.

Subsequently, hypothalami were excised by micro-dissection and submerged in an RNA stabilization solution.

When DHF was used as substrate, samples were mixed with 10 μl of stabilization solution (containing 1 M ascorbate and 100 mM DTT), loaded on a LiChrospher 100 RP 18 HPLC column, 5 μm particle size (Merck, Darmstadt, Germany) and eluted with 5 mM KH2PO4, pH 2.3, containing 7% acetonitrile at a flow rate of 1 ml/min.

For molecular biological investigations, small skin samples (∼1 cm) from 7 men (aged 32 45) and a woman (aged 46) were placed into sterile universal tubes (10 ml) containing RNA stabilization solution, RNAlater, to inhibit RNases.

Total RNA was extracted from tissues preserved in RNA stabilization solution with Trizol (Invitrogen, Carlsbad, CA).