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As expected, the NE-4C untreated cells demonstrated a split percentage of 100%, indicating that all cells entering a mitotic phase resulted in cell division.
Following treatment with either H89 or Wort, cells returned to a split percentage of 0% as seen with WntA only treatment.
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However, treatment of WntA resulted in a significant decrease of split percentage to 0% (p < 0.001), where mitotic cells appeared to become arrested in a round stage denoted in Figure 4D (WntA Image) with black arrows.
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We also show that PGE2 alters the phenotype of Wnt-treated cells, which corresponds to an increase in split percentage.
Therefore, we also quantified the split percentage, defined as the percentage of cells that successfully divided into two daughter cells during the recorded time period.
Split percentage was defined as the percentage of cells that fulfilled the complete cell cycle, which was evaluated based on whether the parent cell could successfully split into two daughter cells.
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