Exact(4)
In response to two electrical pulses (inter pulse interval 50 to 70 ms) delivered at the sciatic nerve electrode, most of the sympathetic efferent fibers exhibited a speeding of conduction; the second action potential was conducted at a faster conduction velocity than the first action potential.
For the presumed sympathetic fibers, a speeding of conduction was observed to the first twin pulse over stimulus interval range of 20 to 100 ms, with slowing of conduction at ISIs of 10 ms and 500 ms (filled circles in Fig. 6A).
For example, a depolarizing after-potential occurs if the outward potassium movement does not quite compensate for the inward sodium movement leading to excess intracellular sodium; this would lead to a "hyperexcitability" of the membrane and a speeding of conduction as seen in the sympathetic fibers.
An important novel finding was that sympathetic efferent fibers in cutaneous nerves exhibit a speeding of conduction to a single pair of electrical stimuli separated by 50 ms; the second action potential was conducted faster than the first unconditioned action potential.
Similar(56)
Using centrifugal recordings in which sympathetics and afferents could be positively identified by stimulation of the sympathetic chain or the dorsal root, respectively, we found that sympathetic efferent fibers exhibited an absolute speeding of conduction to a single pair of electrical stimuli separated by 50 ms; this was never observed in primary afferent fibers.
Previous investigators have suggested that a relative speeding of conduction to twin pulse stimulation develops in C-fiber nociceptors in the presence of activity-dependent slowing [9].
Our finding that only sympathetic efferents show absolute speeding of conduction to a twin pulse (ISI = 50 ms) provides such a criterion.
Similarly, in centripetal recordings, absolute speeding of conduction to a single twin pulse was observed in nerve fibers that were unresponsive to mechanical, thermal or chemical stimuli.
In centrifugal recordings, sympathetic fibers exhibited absolute speeding of conduction
In centrifugal recordings, we observed that sympathetic efferents, but not primary afferents, exhibited absolute speeding of conduction when tested with a single twin pulse (ISI 50 70 ms); the conduction velocity of the second action potential was faster than the first, naive action potential.
In centripetal recordings we used the 60 twin pulses protocol to investigate the relationship of activity-dependent slowing and relative speeding of conduction.
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