Because our measurement error correction methods rely on a correctly specified exposure model, we took care to choose the best-fitting kriging variogram to model our data.
The purpose of this step is to establish an uncertainty distribution for the "typical" human dose associated with a specified magnitude of effect and endpoint, and with specified exposure conditions.
By definition, the "half maximal effective concentration (EC50) refers to the concentration of a drug, antibody or toxicant which induces a response halfway between the baseline and maximum after some specified exposure time.
We generated seven cell populations, designated B1, B2, B3, B5, B6, B7 and B8, each representing cells that remained viable after the specified exposure time point in days.
Cells incubated in the absence and presence of rPA20 were collected by centrifugation at the specified exposure time.
For simplicity, we only specified exposure and transmission within the same room and considered transmission between rooms as part of the general community exposure.
ROI measured with the specified exposure time following the addition of luciferin (Gold Biotechnology, St Louis, MO) at a final concentration of 150 mg/mL in the cell culture media.
By combining the estimated exposure-effect relation with information on exposure distribution for a population of interest, we can calculate the proportions of disease cases attributable to occupational ETS exposure as well as the excess number of cases due to specified exposure conditions.
At the conclusion of each experiment the investigator is supplied with records that document chamber conditions that have been monitored throughout the entire experiment, with estimates of the accuracy that was achieved in creating the specified exposure profile.
