Expression of Lpa in vivo does not affect biotinylation of the E. coli acetyl-CoA carboxylase (ACCase), demonstrating that Lpa is a specific probe for lipoate disruption despite its broad substrate specificity among lipoylated proteins.
Another constraint limiting direct imaging strategy is the necessity to develop a specific probe for each molecular target and then to validate the sensitivity, specificity, and safety of each probe for specific applications before their introduction into the clinic.
Accordingly, annexin V, which binds specifically to phosphatidyl serine residues has been used in this manner as a specific probe for apoptosis (Koopman et al. 1994).
PES was measured by both a specific probe (Gaeltec probe) and by the feeding tube after mobilization (PES-FT).
In this technique, an optimal limitation can be achieved through a specific probe excitation, provided the amount of second harmonic that must be compensated is previously quantified.
From Aspergillus niger a 1.9 kb DNA fragment containing β-Xylosidase gene (xlnD) was generated and used as a specific probe (Perez-Gonzalez et al. 1998).
SYBR Green I real-time PCR relies on detection of a fluorescent signal using the fluorescent chimeric dye SYBR Green I, eliminating the need for a specific probe.
However, in the heterogenous format the recognition of target sequence occurs on a specific probe sequence linked to a solid support.
A specific probe for the detection of L. brevis was designed and no specific PCR products were amplified, as verified by the cycle threshold value.
Using the transient absorption signal at a specific probe delay as feedback, the triplet signal could be optimized over the singlet contribution.
Other molecular techniques proposed for fungal identification such as the use of hybridization with a specific probe and the specific nested PCR [14, 16] could be useful to confirm a specific fungal infection.
