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These designer nucleases specifically introduce a double-strand DNA break (DSB) at a specific locus recruiting the DNA repair machinery to this site.
Together, the findings suggested that M2_3_22 could be introduced as a specific locus in the HLA-DRB1 gene region for linkage analysis and disease association studies.
Here we visualize within a living cell the pore-size dependent deformation of a specific locus engineered into chromosome-1 and cleaved.
With this method, many single copies of a specific locus were analyzed, each with 3D spatial precision on the order of 10 nm at a rate of 10 Hz.
Induction of transcription is an active and targeted process that needs demethylation of a specific locus by enzymes.
Whitehead maintains that events in the world have a specific locus with reference to God, but God has no locus with reference to the world (Johnson 1983, 9).
This method provides accurate estimates of the number of molecules of a specific locus.
At present, resequencing on a substantial scale needs to be targeted to a specific locus [26].
ZFRs could be used to promote transgene integration at a specific locus, or deletion of specific genomic DNA segments.
Mutant isolation has traditionally been followed by time consuming mapping procedures that localize the experimentally induced region to a specific locus.
Segments of each serovar genome were sequentially searched until a specific locus was identified that did not show homology with other salmonellae or non-salmonellae genomes in NCBI.
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