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In 1976, Khaw et al. [ 36] reported a specific localisation of a purified antibody against cardiac myosin.
Mutations did not appear more frequently over the course of disease nor was its occurrence associated with a specific localisation of metastases.
In Barrett's carcinoma (figure 6Di and ii) LGR5 mRNA shows a specific localisation at the bases of the malignant glands (n=5).
A localisation was confirmed by capture of images of at least 10 metaphase spreads on which a specific localisation could be observed on two X chromatids or at least three chromosome 5 chromatids.
Finally, a specific localisation has been postulated as a prerequisite for optimum photosensitisation, suggesting that photosensitisers may have different PDT mechanisms at different time points (Star et al, 1986; Henderson and Bellnier, 1989).
The cells extracted 24 h after injection of m-THPC showed a significantly higher PDT effect than those extracted after 2 h, whereas in vivo the effects are relatively similar, indicating either more m-THPC present in the cell or a specific localisation within the cell.
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Hybridisation with a sense probe resulted in a diffuse background and did not show any specific localisation (data not shown).
The method provided a highly specific localisation of the HMFG1 epitope to the ciliated and 'ciliogenic' cells of the endometrial surface.
Therefore both staining patterns were evaluated separately to identify a possible cell specific localisation of EGFR and to evaluate an association of a specific pattern with prognostic pathological parameters.
To assess whether proteins scored as hits in our screen have a direct or indirect role in the organisation of the tER-Golgi units and whether there is a correlation between specific localisation and the BC phenotype, we expressed 6 C-terminally V5 tagged hits and examined their cellular localisation as well as any dominant negative effect on the tER-Golgi unit organisation (Table 3).
Studies with a conjugate of carboxypeptidase G2 (CPG2) and the F ab' 2 fragment of monoclonal anti-CEA antibody, A5B7, have shown specific localisation in a human colon tumour xenograft, LS174T, growing in nude mice.
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