Here, we show that, in the skin, PPARβ/δ induces a specific IL-1 signalling "module" both in human psoriasis and in PPARβ/δ transgenic mice.
Recently Proteinase 3 has been identified as a specific IL-32α binding protein which cleaves the cytokine to enhance its activity [24].
IL-6 exerts its action via a specific IL-6 receptor (IL-6R).
Culture supernatants were harvested for further analysis using a specific IL-6 ELISA BD Biosciencess, Allschwil, Switzerland).
IL-10 serum levels were also measured using a specific IL-10 ELISA kit, BD Biosciences, San Diego using a human recombinant IL-10 provided with the kit.
Cells were then stimulated with fHAs (20 μg/ml) for 18 hours and culture supernatants harvested for further analysis using a specific IL-6 ELISA BD Biosciencess).
In a cross-sectional sample of patients with established RA, the magnitude of the in vitro effect of IL-4 was lower and was not associated with a specific IL-4R allele.
Thus, NF-κB activation in SZ95 sebocytes seems to be a specific IL-1-mediated event, indicating a major role of IL-1 in sebocyte inflammatory processes, as previously shown.
Only 3 out of 14 patients showed a specific IL-17 secretion upon stimulation with PPD, but these patients showed no clinical signs correlating with the increased IL-17 secretion.
Cells were then stimulated with fHAs (20 μg/ml) or Pam3CSK4 (25 ng/ml) for 18 hours and culture supernatants harvested for further analysis using a specific IL-6 ELISA BD Biosciencess).
The biological activities of IL-4 are mediated by a specific IL-4 receptor (IL-4R) that is expressed at densities of 100 5000 copies cell−1 (Nelms et al, 1999).
