Total GSH was measured using the Bioxytech GSH-420 assay kit, which uses a method based on the formation of a chromophoric thione by specific elimination of GSH-thioether; the absorbance measured at 420 nm is directly proportional to the total GSH concentration.
These results suggest that TSC2 could be a target for the specific elimination of Rb-deficient cancer cells, which are frequently found in human cancers.
Determination of total GSH was based on the formation of a chromophoric thione by specific elimination of GSH-thioether, and the absorbance measured at 420 nm is directly proportional to the GSH concentration.
Very recently, an alternative method for specific elimination of mutant mitochondrial genomes was reported, utilizing mitochondrially targeted TAL-effector nucleases (mitoTALENs) (Bacman et al, 2013).
A promising example is the specific elimination of human immunodeficiency virus type 1 (HIV-1) infected cells by LTR-driven suicide genes.
These results show that specific elimination of GRP94 triggers a regulatory circuit to enhance expression of specific ER chaperones which include GRP78, CNX and CRT.
Further, the high variability of conductivity suggests that its level in EBC is not regulated by a specific biological elimination mechanism, but depends on a random process, that would make its use as a normalisation factor hazardous.
The development of novel approaches for specific elimination of autoreactive plasma cell memory remains a challenge for the future [ 74, 75].
Our study, together with a previous study, highlights the significant potential of mitoTALENs for the specific elimination or correction of disease-relevant mtDNA mutations that are responsible for mitochondrial diseases in humans.
As PI-9 expression correlates with an unfavorable clinical outcome, our targeted treatment approach using the here-described mutant GrBs may lead to specific elimination of these resistant cells, resulting in an improved therapeutic effect.
