Segmentation looks for changes in genomic abundance, not regions of a specific copy number state, enabling segmentation to be highly effective in cases where tissue heterogeneity can lead to non-integer copy number intensities.
So the second little innovation, which I think is very clever, is simply that when you submit a game for sale, you get a unique URL that you can spread on your own, which is linked to your specific copy of the game.
Based on available sequence information and suitability of priming sites, primers for the first round of PCR were designed to amplify both copies of Ara h 2, both copies of Ara h 1, or one specific copy of AhFAD2.
Those randomly assigned to the intervention condition were provided with the web address and a unique password to a study-specific copy of the CYD.
In yeast, Mlp2p is the result of the specific genome duplication; however, a spindle organizer-specific copy of Tpr homologs has been independently reinvented several times in evolution for reasons that are still unclear (Jimenez et al. 2000; DeGrasse et al. 2009).
These corresponded to the loss of specific copies of the DAZ, CDY1 and BPY2 gene families.
Based on alignment scores and the presence of unique exons, we were able to unequivocally assign two clones to specific copies of YTHDC2 (Additional file 2: Figure S2B).
At least 20 clones of each PCR product were sequenced, and all the primers used could amplify the specific copies of OpdCYC and OpdcyclinD3 genes.
Some specific copies of genes (PAL, CHS, IFS, F3H) in the phenylpropanoid pathway were near or falling into these quantitative trait loci by browsing the reference genome sequence of Williams 82 (http://www.phytozome.net/soybean).net/soybean
Additionally, although a LINE element was identified as being methylated (initially isolated using affinity chromatography and an anti-5-methyl cytosine antibody) attempts to identify a specific genomic copy of the LINE element that is methylated in E. histolytica have been unsuccessful to date [ 52].
