In conclusion, we first showed that carotenogenic enzymes can be targeted into the specific cellular location of recombinant hosts and this targeting strategy can serve as the basis for the subsequent development of sophisticated pathway engineering in microorganisms.
In spite of these results, there was no significant enrichment of specific cellular location (data not shown), indicating that the aging-related cellular activities might take place in various cellular compartments, rather than specific locations.
To give a more complete picture and greater accuracy in the identification of specific pathways involved in prion pathogenesis, we annotated the PRG list based on information on the specific cellular location of the expression of individual genes.
Nor is it possible to determine the specific cellular location of the signaling pathways, i.e. if they are strictly intravascular or whether they involve the parenchymal cells as well.
To date, more than 200 PIPs have been identified, most of them having the consensus PPP1 binding motif (PPP1BM) RVxF that binds to the catalytic subunit of PPP1, determining its function and specific cellular location (Fardilha et al., 2010; Hendrickx et al., 2009).
In another variation of the same theme, the catalytic subunits of the protein phosphatase 1 (PP1) contain docking sites that host short linear motifs (SLiM) of regulatory subunits that in turn direct the phosphatase to specific cellular locations and determine substrate specificity [16].
So far the kinases that have been tested on myosin VI are members of the PAK family, which exist as a number of isoforms with specific domains that target them to specific cellular locations.
The formation and delivery of transport intermediates to specific cellular locations are complex processes that can be divided into several stages [1].
Expression of dmrt1 was observed in specific cellular locations in males of the bisexual ancestor S. pyrenaicus (PP) and both in nuclear non-hybrid males (AA) and diploid hybrids (PA) of S. alburnoides (Figure 5a, b and c).
The mechanism by which RNP granules self-organize at specific cellular locations is currently poorly understood.
