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As a source of starting material, we used either embryonic day 14.5 (E14.5) mouse embryonic fibroblasts (MEFs) or mouse adult ear skin fibroblasts (MAFs).
They are utilized mainly as a source of starting material in the pharmaceutical and food industries and show numerous biological activities of interest, for example, antioxidant capacity, anti-inflammatory action and stimulation of the immune system [ 33, 34].
Due to the availability of a large quantity of A. chrysantha and because it also had abundant PPARγ-binding activity, this species was chosen as a source of starting material for the isolation of PPARγ-binding ligands in fruit of the Actinidia genus.
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Genuine businesses – such as restaurants, property firms, antiques importers, nail salons, barber shops and taxi offices – are utilised as a source of start-up capital and as a way of cleaning tainted cocaine cash.
While DNA derived from a subject's peripheral whole blood is a preferred source of starting genetic material, continued access to the participant for additional venipuncture may not be possible, or DNA isolated from peripheral whole blood may be available in limited quantities.
Mapping of binding-induced changes between the E2·E3BP core and PDK1 and PDK2 creates a rich source of starting points to better define critical regions that could serve as targets for future drug design.
Since most biomarkers used in clinical screening are blood based, it has been suggested that maternal plasma obtained in the first trimester could be a good source of starting material [116].
Since murine NSCs have been "primed" with several of the factors originally discovered to reprogram fibroblasts into iPS cells, they represent an attractive source of starting material for iPS cell induction studies.
We have recently successfully extended the reconstitution and fractionation studies described here to detergent-extracted ER membrane proteins from rat liver, enabling purification from a more abundant source of starting material.
Finally beyond metabolism, our algorithms could also be utilized in the context of chemical synthesis to enumerate all the possible routes linking a target molecule to a source set of starting reactants, enabling the search for the best routes in terms of production costs.
The call rate did not depend on the source of starting genomic DNA, as call rates from 8 freshly prepared lymphocyte samples were also 98.2% on average in the investigator's laboratory.
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