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Moreover, altering genes in the worm inactivating them or introducing human ones can be as simple as dipping the animal in a solution of DNA's molecular cousin RNA.
The researchers spiked a solution of DNA molecules with a dye called YOYO-1, then applied the mixture to a silicon-based mold riddled with channels just a few micrometers wide.
A solution of DNA (16 μm long - 48,502 bp; Bio Basic Inc., Markham, Ontario, Canada) was prepared in a concentration of 10 ng/μL with TE buffer (10 mM tris-HCl and 1 mM EDTA, pH 8.0).
Therefore, for high resolution structural investigations the titrations are usually carried out in the opposite way: Protein into a solution of DNA (or RNA) to prevent this aggregation phenomenon.
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A solution of plasmid DNA containing mouse BMP-4 (pMiw-BMP4) was injected into the gastrocnemius of BALB/cA mice, and electric pulses were applied through paired-needle electrodes inserted percutaneously.
While inserting a cochlear implant into the animals, the team injected the cochlea with a solution of BDNF DNA, then used electrical pulses from the device to create pores in the cells lining the cochlea and coax the DNA to enter the cells.
A solution of CT-DNA was made with 10 mM Tris-HCl buffer of pH 7.4 and its purity was verified by its absorbance at 260 and 280 nm.
Results obtained with a gradient of decreasing amount of methylated control DNA diluted into a solution of unmethylated control DNA indicate that even 5%% of methylated DNA can be reliably detected.
Films with thicknesses in the range of 0.7 2.5 μm are produced by spin-coating (500 700 rpm) onto quartz substrates (1 × 1 cm) a water solution of DNA and R6G, with a 1% of R6G/DNA weight ratio.
The computer, a liquid solution of DNA and enzymes, was programmed to detect the kind of RNA (a DNA cousin) that would be present if particular genes associated with a disease were active.
Even so, many biochemists argued that the unnatural laboratory conditions--the researchers had to crystallize a concentrated solution of DNA to detect the unusual structure--could explain the four-stranded configuration.
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