The platinum content of the sample was determined by comparison with a platinum standard which was prepared by dilution of a standard solution of platinum of defined concentration.
We then prepared a 6nM POM2 scFv solution and mixed it with a series of defined concentrations of the epitope-mimicking peptide (13 different concentrations, ranging from 100-0.01nM 100-0.01nM
In the study, PGS and PGS/Bioglass® composites were subjected to enzymatic degradation in tissue culture medium or a buffer solution at the pH optima in the presence of defined concentrations of an esterase.
Solutions of a defined concentration of reference compounds were tested.
Using a panel of solutions of defined antibody concentrations – ranging from 12nM-125pM – we first created a standard curve of POM2 IgG (data not shown) or scFv (Supplementary Figure S7B).
A defined concentration of H2 in an aqueous solution was prepared by a mixed gas flow of H2 and N2 (or O2) controlled by using a gas flow meter (KOJIMA Instrument Inc).
For this, individual worms were confronted with a defined concentration of the pathogens in NGM solution and their survival checked after 24 h.
The pulsed addition of a highly concentrated substrate solution was repeatedly triggered (about five to seven times during the entire process) whenever the glucose concentration dropped below a defined concentration of several grams per litre (Fig. 2).
You can select from a defined concentration or create your own tailored concentration with the approval of your faculty advisor and the MIT Sloan Undergraduate Office.
Fill your burette with a solution of known concentration that will react with your solution.
If Griggs despaired of a solution, he aptly defined the problem.
