Cells were suspended in 100 μl PBS, and 10 μl RNase A solution was added.
Then, a solution was added dropwise to the initial mixture that contained deionized water (5 ml), hydrochloric acid (3.75 ml), and ethanol (119.35 ml).
Finally, 0.5 mL PI/RNase A solution was added and incubated for 30 minutes.
Traditionally, when painting, mineral pigments are ground into powder and a glue solution is added to make a liquid paint.
After a second wash, substrate solution is added.
Then, an aliquot of a 1% sodium citrate aqueous solution is added.
After a subsequent wash, the tetramethylbenzidine substrate solution is added for color development.
Initially, the required volume of stock drug solution is added to an empty scintillation vial.
After incubation of a conjugate solution, a substrate solution was added.
For the time sweeps, 1 mL of a stock solution was added to a plastic vial containing the required amount of GdL.
He had to wait a few moments while the sample was mixed with chemicals and poured into a test tray where a developing solution was added.
