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We sequenced more than 4×107 tags on a SOLiD sequencing, approximately 70% of which were high-quality sequence tags 35 nt in length.
The PCR products were then sequenced on a SOLiD5500 sequencer (Life Technologies); one quad of a SOLiD sequencing slide was required for each sample.
Each sample was sequenced on a single quad of a SOLiD sequencing slide (Life Technologies).
For each sample, 1 quad of a SOLiD sequencing slide was used.
SOLiD sequencing of ChIP-Seq libraries were performed on an ABI SOLiD4 instrument with 35-bp reads according to manufacturer's instructions (Life Technologies, Grand Island, NY) by the UTSW McDermott DNA Sequencing Core.
Although Pico Profiling also suffered from this compression of dynamic range, it still provides far more true positive measurements than SOLiD sequencing of picograms of RNA (60% versus 43% respectively).
Microbiota gene content was determined by high-throughput SOLiD sequencing of total faecal DNA [ 25].
SOLiD sequencing of four V. vulnificus strains representing different clades (1 and 2) and biotypes (1 and 2) was used for comparative genomic analysis.
SOLiD sequencing of multiple bacterial genomes of V. vulnificus and subsequent comparative genomic analysis identified numerous genes that are common to the most virulent strains yet lacking from attenuated strains for which genomic DNA sequence data are available.
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