Data were collected and analyzed as follows: Three random field images were collected from a single well of an eight-well culture slide under 20 × objective using SPOT software (SPOT, RTke Diagnostic Instruments, Sterling Heights, MI, USA).
They were mounted on gelatine-coated slides, counterstained with cresyl violet, observed under light microscopy (BX60; Olympus Optical, Thornwood, NY) and photographed with SPOT camera and software (Spot Advanced; Diagnostic instrument, Sterling Heights, MI).
Image analysis was carried out using the software Spot, a software package for the analysis of microarray images to extract spot intensity data from 16-bit tiff image files.
Sections were analyzed (NIKON Eclipse E600) and images collected using appropriate software (Spot v.4.5).
The spots that were technically flawed or flagged automatically by the GenePix software, spots with a diameter less than 60 μm were removed from the data of each microarray.
Its tracking software spots where shoppers quit a site and helps Web designers tweak the features accordingly.
The launch software spotted it, too, and did what it was supposed to do in such circumstances: abort engine five and then shut down the remaining eight.
Its software spotted that although customer defections peaked in the 13th month, the decision to leave was made much earlier, around the ninth month (as indicated by things like the number of calls to customer support services).
He averages two job offers a week, to say nothing of an avalanche of 200 e-mail pitches from headhunters that he filters out with a software program that spots telltale words.
aPDQ SSP# is a PDQuest software standard spot number indicating the unique location of each spot automatically assigned on a 2D gel and is essential for comparing the same spots on different gels.
