The system was tested using a small scale screen of protein kinase A (PKA) modulators but in principle can be applied to any assays resulting in a change in analyte charge or size, such as peptide cleavage, dephosphorylation, and deacetylation.
The prototype beams test can successfully serve as a small scale screening test providing insights on materials selection for the full-scale beam tests.
Several PEG/phosphate systems varying in PEG molecular weight, pH and NaCl concentration were investigated in an automated small scale screening performed on a liquid handling station.
We previously identified xav in a small scale genetic screen due to its abnormal swimming behavior and decreased number of neuromuscular synapses[11].
In a small-scale screen, analogues of TE1 were synthesized in the laboratory and PIN2 GFP seedlings were incubated in 25 μM chemical for 120 min to monitor the change in PIN2 localization.
Moreover, following a small-scale screen of salinity response in tomato plants following treatment with individual or combined components of the Alethea compound, no clear synergistic response of combining PDJ, SB and Arg compounds was evident, and there was no significant difference between the differing treatments in this element of our study.
Light microscopic analyses in a small-scale screen with ∼50 flies/genotype suggested that knockdown of cisd2 modified the external eye morphology in two strains that overexpressed wild-type Drosophila palmitoyl-protein thioesterase 1 (Ppt1).
In contrast to large-scale screens that aim to test every gene or protein in an organism, performing a small-scale screen by selecting candidate genes based on prior knowledge can greatly increase the efficiency and reduce the cost of the screening process.
A small-scale screen identified dodecyl maltoside as a useful detergent for protein extraction and yielded a highly active protein.
These vectors in combination with a small scale Escherichia coli expression screen can be used to assess in parallel the effect of fusion tags on solubility.
