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It requires only a small sample volume.
The novel probe head separates a small sample volume of 0.65 ml from the bulk liquid by means of an impeller.
Yielding results within 10 min, the assay requires only a small sample volume taken directly from primary serum tubes, i.e., no pretreatment of the sample is necessary.
In addition the PHB amount can be measured with a preparation time of less than 10 min and only a small sample volume (100 200 µL).
Moreover, CD is also affected by a limited frame rate, allowing low temporal resolution; PW displays the complete spectrum of velocities through the cardiac cycle, but related to a small sample volume and along a single line only.
Contrary to published methods using large sample volumes (about 250 mL–4 L) and time-consuming offline SPE, we were able to quantitate all the proposed hormones using a small sample volume (1 5 mL).
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For the t7 and t14 sampling points of the mixture experiments, the same method was used as for E2 and EE2, consisting of gas chromatography coupled to ion trap detection (GC-ITD) using a Saturn 2200 ion trap detector (Varian model 9095), which had the advantage of requiring a smaller sample volume because of the higher sensitivity of the method.
The optimal MEPS extraction conditions were obtained using C18 phase as a sorbent, small sample volume (20 μL) and a short time period (approximately 5 min) for the entire sample preparation step.
Since a relatively small sample volume is drawn for every point in time (i.e. continuously), time resolution is slightly blurred by sampling from the flow-through cell.
Moreover, they mapped 127 unique glycopeptides mapped to 51 different glycoproteins from a very small sample volume (only 1 μL rat serum) [ 39].
For in vitro assays a bath sonicator was used due to the small sample volume.
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