Most of these Transcriptional Units in both human and mouse present conserved sizeable sequences (>40 bp) overlapping Hox transcripts, suggesting that these Hox antisense transcripts are functional.
We found that 5.5% of the D. albomicans genome consists of repetitive elements (compared to 5.35% of D. melanogaster [ 21], Additional File 4), suggesting that our short-read assembly has captured a sizeable fraction of repeat sequences.
Despite this, a sizeable fraction of 5' UTR sequences have no uORFs at all, including some of those above 400 nt in length when at least three uORFs are expected by chance (see Additional File 2).
This observation led to the hypothesis that, along with SLSs stochastically formed because of sequence composition, a sizeable quota is possibly the result of selective pressure, due to the need to preserve a biological function.
Furthermore, there was a sizeable gap in the predicted sequence of T. castaneum Dscam3 (Additional file 14), the placement of which also does not match the evolutionary relationships among the organisms.
Data sets that had a sizeable contribution (>30) of both IGSP sequences and non-IGSP sequences were aligned with MUSCLE v3.6 [45] and analyzed for recombination signals using the 3SEQ algorithm [33], [46].
The pan-genome of the eight compared P. ananatis strains consisted of a core genome comprised of 3,876 protein coding sequences (CDSs) and a sizeable accessory genome consisting of 1,690 CDSs.
Amplifications were done in both 3' and 5' directions to obtain the complete sequences of these genes, including a sizeable portion of their promoter regions.
From these studies with higher resolving power than previous cytogenetic analyses, we concluded that while early replicating sequences are found predominantly in G-negative bandsizeableeable portion is not.
The presence of genome wide repeats and other repetitive sequence in the mouse and human genomes means that a sizeable proportion of short tags can not be placed uniquely (Faulkner et al., 2008).
