An aliquot of cells (300 μl) was immobilized on the microslide glass (Matsunami Glass, Osaka, Japan) and subjected to a TUNEL assay using an In situ Cell Death Detection Kit, Fluorescein (Roche) according to the supplier's instructions.
For detection of apoptotic cells in whole embryos, a TUNEL assay was performed using an In Situ Cell Death Detection kit-AP (Roche, Germany) according to a previously described protocol [49].
For in situ XRD, an in situ cell was designed with a Be window for X-ray penetration.
To evaluate the extent of apoptosis, a TUNEL assay was performed using an in situ cell death detection kit (Roche, Mannheim, Germany) according to the manufacturer's protocol.
Large zeolite ZSM-5 crystals were loaded into an in situ cell (Linkam FTIR 600) equipped with a temperature controller (Linkam TMS 93).
Apoptosis was examined via the TUNEL assay using an in situ cell death detection kit (Roche) with a modified protocol based on the manufacturer's instructions.
The large zeolite H-ZSM-5 crystals were loaded into an in situ cell (Linkam FTIR 600) equipped with a temperature controller (Linkam TMS 93).
The percentages of apoptotic cells were measured with a FACSCaliber flow cytometry (Becton Dickinson) using an in situ cell death detection kit (Roche Diagnostics GmbH, Mannheim, Germany), according to the manufacturer's recommendations as described previously (Kunisue et al, 2000).
A TUNEL assay was performed for apoptosis detection using an in situ cell death detection kit (Beyotime).
The cells were rinsed in PBS and labeled, using a solution of the label and enzyme solutions from an in situ cell death detection kit, fluorescein (Roche Applied Sciences, Mannheim, Germany), with appropriate controls labeled only with the label solution.
