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Open image in new window Fig. 1 a Sites from where sample were collected in Chittagong City.
Bicistronic transcripts gave identical poly(A) sites from both gene-specific primers (e.g. from A8 and A6, and from ND4L and ND4).
Overall, EST clones provided evidence that 1,155 poly(A) sites from 1,001 genes represented expressed transcript variants (Table 1 and Additional file 2).
Domains were deleted or truncated in transcript variants from 7,641 poly(A) sites from 4,142 genes (Tables 1 and Additional file 5).
In addition, we observed that sequences in a downstream region broader than the DSE discriminate actual poly(A) sites from randomly occurring AAUAAA hexamers.
We estimated the relative processing efficiency (RE) of poly(A) sites based on EST counts, normalized in such a way that the highest EST count of all poly(A) sites from the same gene had a value of one.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com