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However, several observations led us to postulate that PRAMEYs were derived from a single transposition of the BTA17 gene block.
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At the sequence level, we noticed that a single transposition event of the 5′-GUGUUC stretch from the 3′ strand of P15 of the wild-type GIR1 to A120 in J15/3 would restore the topology and the base-pairing scheme found in Azo (Supplementary Figure S4).
The existence of the peak is in favor of our explanation that shuffled ancestral paralogs derive from a single transposition event (first hypothesis) involving only one copy of the ancestral pairs.
Our previous studies suggested that the main contribution of Ds exonization to gene diversity is, based on a single transposition event, providing different splice donors for different reading frames rather than providing genetic messages (Huang et al., 2012).
Only a single transposition male from each vial was analyzed.
Both C. cinctus and O. occidentalis GOs can be transformed in ant1GO by a single event of transposition of trnM.
To understand how our model performs under conditions where transpositional and demographic histories are known, we performed two sets of simulation experiments under the extreme case of a single burst of transposition.
A simple transposition of 5 to the position between 2 and 3 can account for the difference in a single event.
A single G/T transposition within the ZAP-70 coding sequence was subsequently found to segregate with skg disease.
Three are trivial: a misspelling, a transposition of two digits, a transposition of two words.
Phylogenetic analyses of 30 randomly-selected copies of OC1_Das yielded a star topology and a lack of subfamily structure, indicating that OC1_Das amplified through a single major burst of transposition, followed by the accumulation of private mutations in its copies.
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