The phrase "a single transformation of" is correct and usable in written English.
It can be used when discussing a specific change or alteration that occurs once within a particular context, such as in mathematics, science, or processes.
Example: "The experiment demonstrated a single transformation of the chemical compound under controlled conditions."
Alternatives: "one change of" or "a solitary alteration of".
For a single transformation, 40 µl of the cell suspension was used with 10 µl of DNA (10 µg).
We also achieved simultaneous disruption and knock-in of a reporter gene, and demonstrate the applicability of the method by designing lactic acid-producing strains in a single transformation event, where insertion of a heterologous gene and disruption of two endogenous genes occurred simultaneously.
The advantages of plastid transformation for bioengineering are several-fold and include the integration of multiple genes in a single transformation event [ 11- 13], lack of gene silencing [ 14- 16], position effect due to site-specific transgene integration [ 17], and minimization of pleiotropic effects due to compartmentalization of recombinant proteins [ 15, 18, 19].
Moreover, multiplex genome editing for the deletion of spo0A and pyrF was achieved in a single transformation, with an editing efficiency of up to 100%.
However, since the dynamics of the molecule prevents the consideration of a single transformation, we opted for considering a transformation for each site.
These include high-level protein accumulation due to increased foreign gene content in chloroplasts (up to 10,000 copies/leaf cell in tobacco; or 80 copies/cell in Chlamydomonas reinhardtii), expression of multiple genes through a single transformation event, increased transgene containment because of maternal plastid inheritance, as well as a lack of position effects on foreign genes [ 8].
There is a single transformation technique that worked in all of the previously transformed volvocine species, namely particle bombardment.
With this technique, numerous DNA fragments are assembled in vivo via homologous recombination at their ends following a single transformation event, allowing the incorporation of an entire heterologous biochemical pathway in Saccharomyces cerevisiae.
The key difference of our method with respect to most of the above methods is that it considers a sequence of local rigid transformations as opposed to a single transformation or to a global influence of each single assignment.
Any single transformation of a 0 to a 1 or a 1 to a 0 would change the parity of the block and make the error detectable.
