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Finally, 9 of 12 sera positive for the γ-subunit displayed a single reactivity (SLE9, 12, 18, 20, 22, 24, 27, 48 and 64).
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A tumor-state immune signature may be systemic rather than discrete; the immune system can undergo a generalized response in which a pattern of reactivities can be more informative than any single reactivity.
The absence of clear tumor-associated antibodies to a single antigen may have resulted from two factors: the antigens we spotted did not include the hypothetical tumor-specific antigen, and/or our quest was directed to a level of separation that was apparently above the capacity of any single reactivity.
Western blot analysis showed a single reactive polypeptide of apparent molecular weight 99,000 in adult myocardium and liver samples; no reactivity was detected with adult skeletal muscle homogenate.
When the 35 anti-PM/Scl antibody-positive sera were analyzed for their subspecificities (anti-PM/Scl-75 and anti-PM/Scl-100) and for further autoantibodies, only one patient was exclusively positive for anti-PM/Scl-75 and six patients showed single reactivity to PM/Scl-100.
Moreover, multivalent ligands often lack a single group with reactivity orthogonal to other functionality in the ligand.
Lupus begins as a less complicated autoimmune process, often involving a single lupus-specific reactivity.
In other words, the microarray analysis did not detect a single tumor-associated reactivity.
Although IHC was performed with a single antibody (because reactivity of the P4 monoclonal antibody is abolished by formic acid pre-treatment used with these tissues), examination of a number of cotyledons was sufficient to identify positive placentas.
Intragroup reactivity was defined as the reactivity of each class of Ig for each fragment specifically, and intergroup reactivity was the reactivity of a single class of Ig to each fragment.
They help to characterize silicate magmas by a single value describing their reactivity.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com