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We designed a multiplex genotyping assay to detect the CYP3A5⁎3C and CYP3A5⁎6 polymorphisms in a single polymerase chain reaction (PCR) and a single pyrosequencing reaction.
Most of our markers are simple sequence length polymorphisms (SSLPs) and can be rapidly genotyped by an automated system under a single polymerase chain reaction (PCR) condition.
This method uses a single polymerase to assemble and clone multiple inserts with any vector in a one-step reaction in vitro.
Amplification of Prx-specific fragments was performed in a single polymerase chain reaction with Prx primer and adapter primer as described in [50].
Here we have presented a model system that could potentially explain how evolution might account for the rise of a single polymerase strategy.
At lower temperatures evolution leads to a predominance of one form, which would make a founder event resulting in a <img src="http://journals.plos.org/plosone/article/asset?id=info?doi/10.1371/journal.pone.0018881.e048.PNG" class= inline-graphic"/> polymerase more likely than the reverse, but evolution alone cannot explain the convergence to a single polymerase strategy.
Similar(43)
Eukaryotic TLS process is carried out not by a single TLS polymerase but by a set of specialized TLS polymerases recruited at a site of DNA damage, although the extent of their individual participation varies depending on the lesion type.
The mitochondrial DNA polymerase from the fission yeast, Pog1, is likely also a single subunit polymerase.
In eukaryotes, three multi-subunit polymerases interact to complete DNA replication, whereas in bacteria a single DNA polymerase is sufficient.
It was demonstrated that 25 loci were polymorphic and 19 loci could be co-amplified in a single multiplex polymerase chain reaction (PCR) assay and genotyped as a single panel on Applied Biosystems 3500 Genetic Analyser.
The Israeli Group 1, poliovirus type 2 aVDPVs were characterized by multiple lineages, numerous amino acid substitutions in neutralizing antigenic sites, a single intratypic polymerase gene recombination with type1 poliovaccine, no recombinations with clade C non-polio enteroviruses and intermittent isolation patterns.
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