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It's a different story when a town prohibits the stolpersteine, as has happened in Munich, the only big German city without a single plaque.
Usually, progeny viruses generated by co-infection of two distinguishable parental strains are first cloned by selection of a single plaque and then characterised by PCR.
Instead, they were honored by a single plaque, referring to them only as "Mexican nationals".
A single plaque from each plate was selected and plated with the host to establish a genetically homogenous virus population.
Four days following the plaque assay, a single plaque from each virus was isolated and used for a second round of plaque purification in MDCK cells.
After final passage, a single plaque was isolated by three consecutive plaque purifications on MDCK cells, amplified in embryonated chicken eggs, and the LD50 of the H5N2 virus was determined in mice for challenge experiment.
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In the case of ME49, a single plaque-purified clone (i.e. ME49 B7.21-E1) that was FUDRr was further passaged in a cat (cross TX332) to derive the parental clone referred to as ME49 FUDRr.
We isolated a single plaque-purified copy of clone C1 that had evolved on ERA at large population sizes; sequencing confirmed that this clone contained the F176L mutation presumably beneficial for ERA infection.
Our results suggest that small plaques can cluster together, thus forming larger plaques as a complementary mechanism to simple uniform plaque growth from a single initial plaque.
Inspired by these findings, we examined whether one mechanism that might lead to large plaques could be the cluster formation of pre-deposited and newly emerged plaques rather than uniform growth of a single, initial plaque.
For example, a subject with a single pleural plaque and no functional deficit would be scored at 1, whereas a subject with bilateral plaques and effects on spirometry was scored at 3. To further break down the sample sets by disease types would have made the subsets too small for statistical analysis.
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