Sentence examples for a single insert from inspiring English sources
Southern blot analysis suggested that 87.5 % of transformants obtained with this protocol displayed single hybridization bands, indicating a single insert of T-DNA in each of the transformants.
Given the number of inserts (total 42) and discarding false positives, raw p-values were adjusted for multiple testing, which resulted in only a single insert position with significant difference and a p-value <0.01 (Adj. p).
Therefore, we have modified Escherichia coli T7 expression vector pET21a to accommodate ligation-independent cloning (LIC) that will allow easy and efficient parallel cloning of target genes with different His-tag lengths using a single insert.
These VLPs provide a safe and efficient strategy for presenting multiple HIV-1 antigens, expressed from a single insert, to the immune system in a structure that mimics the infectious virion.
Interestingly, even with a single round of genetic selection, we were able to identify a single insert enriched in a third of all 5FU resistant clones.
For example, among 13 miRNA loci (16 miRNAs) with non-CIS inserts within 10 kb, ten loci have a single insert, and three loci have two inserts.
Previously, we studied the exonization effect of a single inserted site by Ds providing 5 splice donor sites (1 for forward and 4 for reverse pattern), which may result in 5 exonized transcripts.
A nanomechanical three-dimensional force photonic crystal (PhC) sensor is proposed in this paper by using a shoulder-coupled aslant resonant nanocavity with a single inserted Si pillar and the sensing characteristics are theoretically analyzed and demonstrated.
For the X chromosome EP screen, individual lines of females carrying a single inserted EP element were individually crossed to GAMAREP males.
A single insert-positive clone (with pMAMneo- gef) was selected for transfection into MCF-7 cells after analysis by restriction-enzyme digestions and PCR to establish reading frame orientation and sequence fidelity.
To recover the maximum number of reads, we grouped the 11-bp and 13-bp tags that matched 12-bp tags with the exception of a single inserted or deleted base with their matching 12-bp tags.
