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All samples were dispensed in one sequence over no more than 10 min using a single batch of cells in suspension.
The three technical replicate experiments were performed on the same day with a single batch of cells.
The hiPSC lines were generated from white blood cells using episomal reprogramming [ 26]. hiPSC-CMs were obtained from a single batch of cells.
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It is well-recognized that the spontaneous cytotoxicity of a single batch of CIK cells in continuous culture varies on a daily basis [ 9], and that the cytotoxicity of CIK cells varies from donor to donor [ 21].
Second, a single batch of embryonic cells can divide indefinitely in the lab, so supply isn't a problem.
Even though all of the tumor implanted mice did develop a tumor, and all tumors developed from a single batch of tumor cells, there were single animals in the Vehicle+Tumor group showing no cachexia.
A single batch of CIK cells in continuous culture was used in all the assays described.
It has long been observed that the cytotoxicity of a single batch of CIK cells in continuous culture varies day by day [ 9].
For this study, a single batch of suspension-cultured cells of Arabidopsis thaliana was used, providing a homogeneous starting material.
As such, an added benefit of having access to a long-term culture model that stably maintains a consistent phenotype and genotype over prolonged periods is that multiple studies or multiple repeat doses can be performed with a single batch of tissues or cells.
German consumer agencies traced the outbreak to a single batch of berries.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com