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Development of a single assay that universally detects SRLV is complicated by the high level of genetic heterogeneity of SRLV, which are grouped into five genotypes and several subtypes [ 9- 18].
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In the final dataset, these scores were removed and the final genotype was dictated by the single assay that produced a clear genotype.
Although there is no single assay that can predict rFVIIa efficacy in trauma, the prothrombin time (PT) is recommended as a minimum.
A single assay (IL-1α) that showed no variation in expression across all samples was considered invariant/uninformative and removed from the extracted data set.
For BRAF, a single assay was designed that detects the activating hotspot mutation p.V600E, which results from the c.1799T>A substitution [35].
Second, the drug activity database was generated using a single assay end point, that is, short-term growth inhibition and cytotoxicity.
We present here protocols for preparing a single assay mixture containing PLG2 that can be used to readily detect femtomole levels of ATP.
Furthermore, because multiple relevant markers can be determined using a single assay, it is likely that gene expression profiling will be more cost-effective than currently used diagnostic and prognostic tests.
It is, therefore, unlikely that a single assay will provide an independent additional value beyond that provided by APACHE II.
As these cis-regulatory sites will differ substantially between cell types, we have to reconsider the idea that a single assay design will be able to serve all studies, and that instead we need to develop cell type-specific assay designs.
Of course, the advantage of using NGS methods is that multiple genes can be studied in a single assay, in contrast to PCR-based assays that evaluate one gene hotspot at a time.
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