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Based on the facts that φA1122 [7], [49], [51] and L-413C [52], [53], [53] can lyse almost all (99.8 99.9%) Y. pestis strains of several thousand tested, we propose a broad-range qPCR method in a simplex format.
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Nelder, J. A. & Mead, R. A Simplex Method for Function Minimization.
In our experience, the simplex format strategy (i.e. based on testing phages and viral pathogens in distinct amplification reactions) proved to be the most simple and cost-effective for routine molecular diagnosis since it does not require the specific development of multiplex reactions and relies on a unique control reaction for DNA viruses and another for RNA viruses.
Reactions were carried out in simplex format in 25 μl containing 200 ng DNA, 1× TaqMan® Universal PCR Master mix (Applied Biosystems), 900 nM each primer and 200 nM probe.
Choose a simple format.
A simplex is called degenerate if it is the degeneracy of another simplex.
Diagnosis of a simplex case by DNA analysis.
Let us denote a simplex of as.
7: A simplex wormhole attack is detected.
An m-simplex is a simplex S such that | S | = m + 1. Definition 1.7 ([9, 14]).
The later assigns a constant n-form 1 to each maximal n-dimensional simplex; a simplex being maximal if it is not contained in a larger simplex.
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