However, taking advantage of the non adhesiveness of the cells this article presents an effective simple cultivation integrated foam fractionation method to produce and in situ concentrate rhamnolipids using a heterologous production strain independent of pH, temperature and defined medium composition with little cell accumulation in the foam.
Use of this simple cultivation strategy yielded a bulk PGA volumetric enzyme activity exceeding 93775 IU L-1 within 17.5 h of induction, corresponding to a productivity of 3907 IU L-1 h-1 (or 5358 IU L-1 h-1, considering the elapsed induction period).
Comprehensive cellular and bioprocess engineering has enabled to produce highly complex aglycosylated IgGs in a simple bacterial cultivation with comparable production level as that of mammalian cells.
It was previously shown that simple cultivation integrated foam fractionation is an effective tool for biosurfactant production e.g. of surfactin using Bacillus subtilis (Chen et al. 2006; Willenbacher et al. 2014, 2015) or HFBII using Saccharomyces cerevisiae (Winterburn et al. 2011).
This led to an unforeseeable effective method to produce and in situ concentrate rhamnolipids via simple cultivation integrated foam fractionation using the heterologous production strain P. putida KT2440 containing a plasmid for mono-rhamnolipid production in a bioreactor.
Besides, the evidence that the endophytic strain grows in simple cultivation conditions much like the classic biocontrol strains is further proof that in nature some microorganisms are facultative endophytes, which can optionally live inside plants and other habitats (Hardoim et al. [2008]).
Like S. cerevisiae, H. polymorpha is characterized by simple cultivation mode in inexpensive growth media, well established genetic tools and experience on industrial cultivation and scaling-up.
Moreover, simple cultivation of splenic MDSCs from 4T1 cell-bearing mice increased the expression of soluble IL-6Rα compared to EMT6 cell-bearing mice.
Because the in vivo immobilized lipase can be prepared easily by simple cultivation and separation of the cells, no additional steps for the purification and stabilization (immobilization) are required and can feasibly be modified by rational de novo design of the enzyme.
Therefore, it may suggest that a simple change in cultivation condition and single genetic modification may not be enough to completely reroute the carbon flux from citric acid production to other organic acids.
