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However, a significant length of time is needed to establish iPS cells, and the efficiency of generating T cells from iPS cells is still not high.
A significant amount of time is needed to apply the methodology.
As suggested by Hu et al., a more prolonged reperfusion time is needed to obtain a significant increase in MDA levels after hepatic I/R damage [ 35].
This study also suggests that the 'Candidatus Accumulibacter phosphatis' clade II/'Candidatus Competibacter phosphatis' fractions in the inoculum have a significant impact on the time that is needed for enrichment of 'Candidatus Competibacter phosphatis' cultures and that a long enrichment period may be needed to obtain highly enriched cultures.
Appalachian Roots – Hemp 2.0 is reintroducing a historically significant crop to the coalfields of Eastern Kentucky at a time when it is needed most, its founders say.
A challenge related to this is that since a significant amount of time and money is needed to maintain the overloaded infrastructure and to design a new one, only limited human and financial resources remain for planning and developing alternative mobility systems [37].
The second disadvantage is that significant expertise and large amounts of data is needed for building a high-quality synthesis system which is costly and time-consuming.
Moreover, this is a time consuming process and significant knowledge of both technological as well as economic aspects is needed in this case.
Another less significant alteration was needed.
We found that there is a significant time and educational component needed to learn to insert, calibrate and assess these devices.
Significant CF Center personnel time and fundraising are needed in order to address medical bills incurred by uninsured Amish and Mennonite patients with chronic disease such as CF. Several options are available.
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