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Similarly, we observed a significant decrease in invasion of HepG2 cells transfected with C2GnT1 shRNA compared to cells transfected with a control vector, (mean number of cells/field = 14 versus 99, p<0.0005) (Fig. 6B, E, and F).
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Invasion assays with siRNA pretreated cells resulted in a significant decrease in invasion with an average of 35% /65% /60%spectrin/adducin/p4.1 RNAi treated) invasion as compared to controls (Figure 3d spectrin and S22 adducin/p4.1).
A significant decrease in invasion was seen with both the MDA-MB-231 and MDA-MB-436 cells.
Downregulation of PDGFRα in both cell lines resulted in a statistically significant decrease in invasion of approximately 70%, while PDGFRβ knockdown inhibited invasion by approximately 40% (not determined to be statistically significant).
At a 1 10 HUVEC:U87 ratio, there was a significant decrease in the invasion of HUVEC co-cultured with U87- hHSS1 cells compared to HUVEC co-cultured with U87-pcDNA3.1 U87-pcDNA3.1
A significant decrease in the invasion ability of cancer cells confronted with IL-10-stimulated macrophages was observed upon EGFR silencing, even when comparing with AGS cells transfected with Lipofectamine only.
Similar to the siRNA experiments, there was a significant decrease in migration and invasion of the 231 cells transduced with shGli1 231-shGli11) after 24 h (P < 0.001 for migration and invasion, ANOVA) (Fig. 6a, b).
In sh-scr cells, the absence of SipA, SipC, SptP, or SopE/SopE2/SopB/SipA leads to a significant decrease in Salmonella invasion.
Furthermore, transfection of 22Rv1-VO and 22Rv1-B4 cells with the ITGB8 siRNA resulted in a significant decrease in Matrigel invasion.
We previously showed a significant decrease in matrix invasion after AFAP1L1 knocking-down in U2OS cells 11.
This is consistent with previous experiments where PKCα inhibition also resulted in a significant decrease in cell invasion potential.
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