Several independent experiments showed a significant increase in confluence after addition of ACh (10 μM; Figures 1a and b).
Indeed, when the AChE inhibitor huperzine A (HupA; 10 μM) was added to ACh, a significant increase of confluence resulted after 12 and 24 h.
After having maintained EPCs in culture for four days after confluence, we observed a significant increase in p16INK4A expression levels with typical nuclear localization (Fig. 2B, 2C,).
The trend to use them in nontraditional situations may be a result of a confluence of factors, including a significant increase in deal-related litigation.
A confluence of fortunate events and a significant historical mistake contributed to the consignor's happy payday.
Culture is the general expression of humanity, the expression of its creativity...Europe's diverse cultures, its history and geography are a significant source of its creativity... (And) at the confluence, Europe increases its creativity and innovation potential.
In this region, the river bottom exhibits a significant break of gradient: within 10 miles of the confluence, the depth of the water increases from about 80 feet (25 metres) to 1,145 feet.
When the saturation density (confluence) of the cultures was reached, a significant reduction of the cell coupling was observed (48% of the value found in the proliferating counterpart).
Kindergartens and nursery schools expanded slowly during the 19th century and experienced a significant increase only in the mid 1960s and early 1970s when a confluence of factors led to the significant expansion of both program types.
"We do not anticipate that it will have a significant impact on American Apparel's productivity because of the confluence of several factors including the slow economy and high preexisting inventory levels," he said.
The confluence of increased cardiovascular risk and a failure to be diagnosed by the conventional approach of clinic BP measurement makes masked hypertension a significant public health concern.
