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The response of L. monocytogenes to low pH involves the alternative sigma factor SigB (σB) and is characterized by the synthesis of a number of proteins to mount a significant acid tolerance response (ATR).
The influx of H+ into the parasite via the Na+-ATPase would constitute a significant acid load, which would be countered by the H+-extruding V-type H+-ATPase.
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When sodium decanoate was titrated alone, there was a clear phase of significant acid consumption between pH of around 8 to 6.
Thus it seems reasonable that the differences in the final OD can be explained either by a reduced cell growth, caused by too low ammonium concentrations, with normal fatty acid production or by a maximal cell growth but without a significant fatty acid production in the case of ammonium concentrations higher than 0.4 g l-1.
The different effects observed for SIG and BE imply a significant unmeasured acid load influencing mortality.
For missense variants the evidence for pathogenicity might include conservation across species and a significant amino acid substitution.
However, we found a significant amino acid change associated to a base change in ND2 (5768 G/A), which is located in the TM7 in Atlantic salmon.
In RB1CC1, a significant amino acid shift is predicted, namely R1514C, with a high Miyata score of 3.06; this results in a positively charged residue being changed to a hydrophilic residue.
It was shown that polyhydroxyalkanotes (PHAs) production by this strain, using cheese whey, was low due to a significant organic acids (OA) synthesis.
For example, as the precursor of linoleic acid, oleic acid exhibited a significant negative correlation with linoleic acid (Fig. 3).
Radioactive uptake assays using 14C-labelled ascorbic acid showed a significant increase of ascorbic acid uptake into the brain after stroke.
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