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After removal of the bone and stria vascularis, the sensory epithelium (organ of Corti) was picked away with a sharpened needle and further dissociated by trypsin (0.5 mg/ml) for 2 3 minutes with shaking.
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The insects were challenged by pricking with sharpened needles, which had been previously dipped into concentrated cultures of microorganisms.
The pages, which have text on both sides, can be turned using a sharpened metal needle.
Eye removal was performed with a sharpened tungsten needle in 1 dpf embryos as described in Zhang and Leung [ 33].
A sharpened tungsten needle (CUY614T; Unique Medical Imada) was used as a cathode, which was inserted into the lumen of the right OV.
The tailbud region was then microdissected using a sharpened tungsten needle and care was taken to remove the endoderm and the ectoderm.
These lamellae are then lifted from the bulk, using a sharpened glass needle, and placed on a platinized magnesium oxide substrate, which had previously been patterned into a capacitor structure.
First, lamellae were cut which were centered on a grain boundary, using a Focused Ion Beam (FIB, FEI200TEM FIB) following a method outlined by Schilling et al. 18 Once the FIB had been used to cut a lamella free, a sharpened glass needle was used to lift the lamella clear of the bulk ceramic and place it on a holey carbon (TEM) grid.
It's helpful to sharpen needles before putting them back so that they are ready for the next time that you need them.
A fresh start with a sharpened mind.
By heating and vertically pulling a glass rod (NARISHIGE G-1000) of a 90-mm in length and 1-mm in diameter, we made a sharpen glass needle which has about 23-mm in length, 1-mm in diameter, and less than 1-μm in diameter of tip of end effector.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com