Fig. 3 Relative gene expression of OsXTH2 (OsXTH93270), OsXTH11(Os06g48160), OsXTH11 (Os06g48160), and NAD(P H-dependent oxidoreductase (Os10g02390) in a set of NILs segregating for the P H-dependent
In our laboratory, a number of genes (Bph3, Bph6, Bph9, Bph14, Bph15, Bph10, Bph18, Bph20, QBph4, QBph3) were individually incorporated into 9311 (an elite variety in China) using MABC, and a set of NILs was developed with enhanced BPH resistance.
In root RNA samples taken from a similar experiment, expression patterns detected for three xyloglucan endotransglucosylases (XTH) and a NAD(P H-dependent oxidoreductase (see Table 8) were indeP H-dependentfirmed foxidoreductaseLseeegregaTablefor the Pup1 locus (NILs 6-4, Y-4, 14-4 for +Pup1; NILs 26-6, 28-10, and Nipponbare for −Pup1:).
Validation of the Glu-A3a allele-specific markers with different wheat varieties, a set of NILs and 4 RILs.
Notably, a set of NILs and reciprocal NILs have been developed that contain single (Rphq2, 3, 4) or combined (Rphq2 +3) introgressed segment(s) carrying resistance and susceptibility QTL allele(s) that were identified in an L94 × Vada RIL population [ 11, 16, 17].
A set of 13 BPH NILs, (NIL-BPH2, NIL-BPH3, NIL-BPH4, NIL-QBPH4 + QBPH6, NIL-BPH9, NIL-BPH10, NIL-BPH17, NIL-BPH18, NIL-BPH20, NIL-BPH21, NIL-BPH25, NIL-BPH26, and NIL-BPH25 + BPH26) and the BPH13 donor line were developed and maintained in the Novel Gene Resources (NGR) laboratory, Plant Breeding Division of IRRI (Jena et al. unpublished data).
A set of 92 NILs was obtained through ABRC.
First, a set of ten NILs, recombinant over the long arm of linkage group one, was challenged by mechanical inoculation.
Furthermore, our companion study that evaluated the same set of horticultural traits in a set of sub-NILs for a chromosome 5 region introgressed from S. habrochaites also discovered closely linked QTL for single traits (Haggard et al. 2013).
We sought to validate the existence of SAM_height_6, a strong-effect QTL (16% variation explained, LOD 9.25) on chromosome 5 at 892.81−895 cM (Table 3 and Table S5) using a set of three NILs (Eichten et al. 2011).
