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A set of multiplex PCR primers was designed based on the dnaJ gene of Vibrio species and was employed in a multiplex PCR procedure, then PCR products were subjected to HPLC analysis with PS-DVB-C18 particles DNASep column in which the unique HPLC profile generated from each specific amplicon represented corresponding Vibrio species.
We estimated the fetal fraction via a set of multiplex amplified Taqman assays that target diverse single nucleotide variations (SNVs) to seek positions where the mother is homozygous and fetus heterozygous.
In order to improve the diagnosis of diarrhoeagenic E. coli (DEC), we developed a set of multiplex TaqMan real-time PCRs designed to detect the respective pathogens from an overnight stool culture.
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Finally, a set of 11 multiplex panels containing the 30 most informative markers was validated and proposed for B. ruziziensis genetic analysis.
A set of 11 multiplex panels for the simultaneous amplification of the 30 most informative markers (ranked by their Polymorphism Information Content) is made available.
Here we apply parent-of-origin linkage analysis to the genome-wide SNP data recently reported by Weiss et al. in a common set of multiplex autism families [23].
Depending on the sample size and number of SNPs to be analysed, medium- to high-throughput assay platforms such as BeadXpress and GoldenGate assays from Illumina Inc. with a varying set of multiplexes (96, 384, 768 or 1536 SNPs per assay) are available.
BART uses a set of 12 reactions comprising 11 multiplex PCR reactions containing 9 to 14 amplicons per multiplex, and 1 contamination detection reaction.
Following a parallel double PCR amplification, which employed universal primers in a singleplex reaction and a set of species-specific primers in multiplex, detection was performed in a cost effective and highly specific analysis.
MPEG coder can multiplex a set of independently-coded, arbitrarily-shaped video objects and transmit it through either fixed or variable rate channels such as internet, wireless or satellite communications.
Each set of multiplex PCR primers were designed to give a logarithmic progression of DNA product sizes, ranging from 200 bp to 650 bp in length, which gave regular spacing of marker bands when products were size-separated by agarose gel electrophoresis.
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