Two proteins, nanos and dead end, were targeted, each specific for a set of constructs, and a third related, but non-targeted PGC protein, vasa, was assayed.
Each factor grouped a set of constructs highly correlated to each other and weakly correlated to constructs of other factors, thus indicating that the owner-managers under investigation had valued managerial functions and roles in four different ways.
To test this idea, a set of constructs were made, which contained membrane bound-GFP (M-GFP as gene X) flanked by lox2272 sites, mCherry (gene Y) flanked by loxP sites, and a spacer in unit A of various lengths.
To test if the observed excess energy transfer was unique to the VCV construct with two acceptors, or represented a more general case when multiple acceptors are present, a set of constructs was generated to investigate FRET between a single donor and 3 acceptors (Fig. 3).
To determine whether the potential uORFs in HIC 5'-UTR are involved in inhibition of its translation, we mutated the initiation codons (AUGs) of the uORFs, rendering them non-functional for initiation of translation in a set of constructs encoding the HIC 5'-UTR fused to a FFL reporter gene.
To test the roles of different HATs on this crippled origin, we co-transformed DFΔgal4 cells with pARS1/-B23/G24 plus a set of constructs expressing GAL4-fusions of Gcn5p, gcn5p KQL) (a GCN5 mutant lacking HAT activity), Sas2p, Sas3p, Esa1p and Hat1p, respectively, and measured the stability of pARS1/-B23/G24.
What this produced, as Moi explains, was a set of constructed "ideals" of love, fidelity, self-sacrifice and so on, that constricted and deformed many human lives and selves.
This led to the construction of a set of summative constructs, each paired with testable propositions.
"The problem is that you have a society, a culture, with a set of mental constructs to deal with the world," he said.
A set of new constructs are proposed which compose the uniform behavior modeling profile.
The new method employs a set of geometric constructs that can be used in conjunction with any volume-tracking scheme.
