Two proteins, nanos and dead end, were targeted, each specific for a set of constructs, and a third related, but non-targeted PGC protein, vasa, was assayed.
Each factor grouped a set of constructs highly correlated to each other and weakly correlated to constructs of other factors, thus indicating that the owner-managers under investigation had valued managerial functions and roles in four different ways.
To test this idea, a set of constructs were made, which contained membrane bound-GFP (M-GFP as gene X) flanked by lox2272 sites, mCherry (gene Y) flanked by loxP sites, and a spacer in unit A of various lengths.
To test the roles of different HATs on this crippled origin, we co-transformed DFΔgal4 cells with pARS1/-B23/G24 plus a set of constructs expressing GAL4-fusions of Gcn5p, gcn5p KQL) (a GCN5 mutant lacking HAT activity), Sas2p, Sas3p, Esa1p and Hat1p, respectively, and measured the stability of pARS1/-B23/G24.
To test if the observed excess energy transfer was unique to the VCV construct with two acceptors, or represented a more general case when multiple acceptors are present, a set of constructs was generated to investigate FRET between a single donor and 3 acceptors (Fig. 3).
To determine whether the potential uORFs in HIC 5'-UTR are involved in inhibition of its translation, we mutated the initiation codons (AUGs) of the uORFs, rendering them non-functional for initiation of translation in a set of constructs encoding the HIC 5'-UTR fused to a FFL reporter gene.
A set of utility weights for a set of constructs can then be combined into a single utility score by using multi-attribute utility theory (MAUT).
Our initial task was to assemble a set of constructs in which a gene of interest was fused to a target sequence at the beginning of the gene.
Although some concerns have been expressed towards the use of parcels, it can well be justified when the goal of a study is to understand latent structure of a set of constructs rather than the structure of a set of items (Little et al., 2002).
With more than 2,000 pictures, he moved the figures from one image to another in Photoshop, reimagining the domestic tableaus in a set of final constructed photographs from which he made this series of paintings.
